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Project Researcher Engineer with expertise in in vitro protein biochemistry, purification, reconstitution and engineering (Técnico Laboratorio)

hace 4 días

Barcelona

We are seeking a highly motivated project researcher engineer to join the Biolab Team, with full-time dedication to the Photon Harvesting in Plants and Biomolecules group led by Prof. Dr. Nicoletta Liguori. ¿Tiene las siguientes habilidades, experiencia e impulso para tener éxito en este puesto? Descúbralo a continuación. The successful candidate will primarily work within Nicoletta’s group, while maintaining close integration with the Biolab team and contributing to shared activities and collaborations whenever time and expertise allow. The project research engineer will report directly to Prof. Dr. Nicoletta Liguori and will play a key role in experimental design, execution, and laboratory management, supporting both scientific output and infrastructure development. Key Responsibilities: • Design and execution of cloning workflows, from construct design through verification and expression testing., • In vitro reconstitution of recombinant membrane protein complexes and their biochemical and spectroscopic characterization (e.g., fluorescence and absorption spectroscopy)., • Isolation of membrane proteins from biological materials, including purification of thylakoid membranes from plants, and purification of protein complexes using biochemical and chromatographic techniques., • Preparation and optimization of samples for optical and biophysical measurements., • Management and maintenance of laboratory equipment and infrastructure, including: Planning, evaluation, and purchase of new instruments; Ensuring proper calibration, maintenance, safety compliance, and documentation; Laboratory management and protocol documentation., • Implementation, optimization, and standardization of experimental protocols., • Collaboration with, and training of, researchers from different backgrounds and levels of experience to ensure reproducible, high-quality experimental results. The ideal candidate will have: • PhD in biochemistry, biotechnology, molecular biology, or a related field, • Proven experience in the independent design and production of recombinant proteins, including primer design, molecular cloning (e.g., Golden Gate or similar), transformation (procaryotic and eukaryotic systems), expression, and purification, • Experience with construct verification (restriction analysis, sequencing) and protein validation assays such as SDS-PAGE and Western blot, • Hands-on experience in protein expression and purification from E. coli or other heterologous systems, • Proficiency with FPLC systems and purification techniques such as affinity-tag purification, ion-exchange, and size-exclusion chromatography, • Experience in protein characterization using techniques such as absorption and fluorescence spectroscopy, dynamic light scattering (DLS), and thermal stability assays, • Ability to isolate membrane systems from biological material and purify protein complexes, including experience with sucrose gradient purification and native/SDS-PAGE analyses, • Advance communication skills in English (spoken and written), • Strong organizational and record-keeping skills, with the ability to support multiple research projects, • Knowledge of physiology of photosynthetic organisms., • Experience in the purification and biochemical analysis of chromophore-binding proteins, • Familiarity with protein–lipid micelle formation or reconstitution in lipid bilayers, • Experience in HPLC analysis of small bioactive molecules (e.g., cofactors, chromophores, or other light-absorbing compounds), • Competitive salary according to the institutional salary scales., • Contract for periods of 2 years renewable upon existence of funding or extension of tasks. APPLICATION PROCESS Required application materials: • A presentation letter outlining your interest in the position., • A curriculum vitae, including full contact information. For formal enquiries regarding the application process, please contact: For project and technology enquiries, please contact:

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