Scientist - Cell-Based Assays
5 days ago
Rahway
Are you an experienced Scientist - Cell-Based Assays with a desire to excel? If so, then Talent Software Services may have the job for you! Our client is seeking an experienced Scientist - Cell-Based Assays to work at their company in Rahway, NJ. Position Summary: We’re seeking a hands-on scientist to execute high-throughput cell-based assays in 384-well format. You will culture and plate cells, dispense compounds using the Echo, run diverse detection readouts, and analyze dose–response data to deliver high-quality EC50 results for discovery programs. Primary Responsibilities/Accountabilities: • Maintain, expand, and plate mammalian cell lines for 384-well assays, • Set up and execute assays with compound dispensing via Labcyte/Beckman Echo, • Run and optimize detection formats (e.g., luminescence, cAMP, HTRF/TR-FRET, fluorescence), • Generate dose–response curves (4-parameter logistic fits) and report EC50/IC50 with QC, • Troubleshoot assay performance, optimize Z’ factors, and document SOPs/results, • Collaborate with discovery teams to prioritize studies and timelines, • Additional responsibilities may include setting up Western blot assays and learning how to run in-cell westerns Qualifications: • BS/MS/PHD in Biology, Pharmacology, Biochemistry, or related field; BS with 5+ years, Masters with 3+ years and PHD with 1+ year of industry or equivalent lab experience., • Proficiency in mammalian cell culture and 384-well assay workflows, • Experience with plate readers and assay platforms (e.g., cAMP, HTRF, luminescent assays), • Strong data analysis skills: 4PL curve fitting, EC50/IC50 determination, and curve QC (e.g., R^2, top/bottom constraints), • Detail-oriented, organized, and comfortable in a fast-paced, high-throughput environment Preferred: • Experience with automation/LIQ handlers, LIMS/ELN, and statistical QC (Z’, CV, S/B), • Familiarity with GraphPad Prism, ActivityBase, or equivalent analysis tools, • Hands-on experience with Echo acoustic dispensing.